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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has actually been noticed in lots of tumors, which results in chromosome instability, and so may well lead to tumorigenesis. This protein was identified to affiliate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a important part in advertising mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually described. Two additional splice variants were being reported but thorough nucleotide sequence info is not really nevertheless accessible.
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Blocking peptides are peptides that bind precisely for the focus on antibody and block antibody binding. These peptide usually is made up of the epitope regarded because of the antibody.
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This manual outlines even further feasible optimization of cellular and immunoassay parameters to be sure the best possible effects are...
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This tutorial outlines even more feasible optimization of cellular and immunoassay parameters to make certain the absolute best success are...
Antibodies certain to the blocking peptide now not bind to your epitope over the goal protein. By comparing the staining in the blocked antibody versus the antibody alone, you can see which staining is specific.
Focus on Information and facts Cyclin E belongs into the extremely conserved cyclin household, whose users are characterized by a remarkable periodicity in protein abundance throughout the cell cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show unique expression and degradation styles which lead for the temporal coordination of each mitotic event. Cyclin E types a posh with and features like a regulatory subunit of CDK2, whose exercise is necessary for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and it is degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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